rabbit polyclonal antibody against agr2  (Novus Biologicals)

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: SATB1 immunohistochemistry staining. A , benign prostate hyperplasia. B , prostatic carcinoma with metastasis. C , prostatic carcinoma without metastasis. D , negative control. Magnification: 400 × .

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Immunohistochemistry, Staining, Negative Control

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: SATB1 expression and clinicopathologic parameters of prostate cancer

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Expressing

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: SATB1 expression is correlated with the invasion ability of prostate cancer cells. A - C : The invasion of LNCaP, DU-145 and PC-3 cells was measured by Transwell chamber. A , DU-145. B , PC-3. C , LNCaP. Magnification: 200×. D : Western blot analysis of the relative protein level of SATB1 in LNCaP, DU-145 and PC-3 cells (n = 3). β-actin served as loading control. *P < 0.05 compared to DU-145 cells. Note that SATB1 antibody could not detect SATB2 protein in the cell lysates.

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Expressing, Western Blot, Control

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: Silencing of SATB1 in DU-145 cells. DU-145 cells were untransfected (control), transfected with pSliencer3.1 control vector or transfected with pSilencer3.1-SATB1 vector for 24 h, 48 h, or 72 h. The protein levels of SATB1 and SATB2 were detected by Western blot analysis (n = 3). β-actin served as loading control. * P < 0.05 compared to cells transfected with pSliencer3.1.

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Control, Transfection, Plasmid Preparation, Western Blot

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: Silencing of SATB1 inhibits the invasion and growth of DU-145 cells. A - D : The invasion of DU-145 cells was measured by Transwell chamber. A, DU-145 cells untransfected. B , DU-145 cells transfected with pSliencer3.1. C , DU-145 cells transfected with pSilencer3.1-SATB1. Magnification: 200×. D : Quantitative analysis of the number of invaded cells as shown in A - C (n = 3). E : The proliferation of DU-145 cells was determined by CCK8 assay (n = 3). The cell proliferation inhibition rate was calculated for DU-145 cells at 24 h, 48 h and 72 h after transfection with pSilencer3.1-SATB1. *P < 0.05 compared to cells transfected with pSliencer3.1.

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Transfection, CCK-8 Assay, Inhibition

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: Reconstitution of SATB1 expression in LNCaP cells. LNCaP cells were untransfected (control), transfected with pcDNA3.1 control vector or transfected with pcDNA3.1-SATB1 vector for 12 h, 24 h, or 36 h. A . The mRNA level of SATB1 was detected by RT-PCR (n = 3). B . The protein level of SATB1 was detected by Western blot analysis (n = 3). β-actin served as loading control. *P < 0.05 compared to cells transfected with pcDNA3.1.

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Expressing, Control, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: Overexpression of SATB1 promotes the invasion and growth of LNCaP cells. A - D : The invasion of LNCaP cells was measured by Transwell chamber. A , LNCaP cells untransfected. B , LNCaP cells transfected with pcDNA3.1. C , LNCaP cells transfected with pcDNA3.1-SATB1. Magnification: 200×. D : Quantitative analysis of the number of invaded cells as shown in A - C (n = 3). *P < 0.05 compared to cells transfected with pcDNA3.1. E : The proliferation of LNCaP cells was determined by CCK8 assay (n = 3). The cell proliferation ratio was calculated for LNCaP cells at 24 h, 48 h and 72 h after transfection of pcDNA3.1 or pcDNA3.1-SATB1. *P < 0.05 compared to corresponding cells transfected with pcDNA3.1.

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Over Expression, Transfection, CCK-8 Assay

Journal: BMC Cancer

Article Title: Differential expression of anterior gradient gene AGR2 in prostate cancer

doi: 10.1186/1471-2407-10-680

Figure Lengend Snippet: AGR2 transcript expression in prostate cancer . Two publically available datasets were used to examine AGR2 gene expression in human prostate samples [ , ]. (A) Dataset was from Yu, et al ., and was generated using Affymetrix U95B Array . (B) Dataset was from Lapointe, et al ., and was generated using a cDNA microarray from the Stanford Microarray Database . For each dataset, normalized data was available from the GEO database. The bars are the relative mean expression value ± standard error of the mean. P < 0.0001 for each multi group comparison (Kruskal-Wallis).

Article Snippet: Slides were incubated with rabbit polyclonal anti-AGR2 antibody at 1:400 (NB110-17780, Novus Biologicals, Littleton, CO) overnight at 4°C.

Techniques: Expressing, Gene Expression, Generated, Microarray, Comparison

Journal: BMC Cancer

Article Title: Differential expression of anterior gradient gene AGR2 in prostate cancer

doi: 10.1186/1471-2407-10-680

Figure Lengend Snippet: AGR2 immunohistochemistry . AGR2 staining was localized to the cytoplasm and membrane of cancer epithelial cells. (A) Tissue samples incubated with concentration-matched non-immune rabbit IgG showed no staining. Shown are representative sections of (B) morphologically normal tissue, (C) PIN, and (D) a representative adenocarcinoma (Gleason grade 7) showing low staining intensity, (E) a representative adenocarcinoma (Gleason grade 8) showing moderate staining intensity, and (F) a representative adenocarcinoma (Gleason grade 6) showing high staining intensity.

Article Snippet: Slides were incubated with rabbit polyclonal anti-AGR2 antibody at 1:400 (NB110-17780, Novus Biologicals, Littleton, CO) overnight at 4°C.

Techniques: Immunohistochemistry, Staining, Membrane, Incubation, Concentration Assay

Journal: BMC Cancer

Article Title: Differential expression of anterior gradient gene AGR2 in prostate cancer

doi: 10.1186/1471-2407-10-680

Figure Lengend Snippet: AGR2 expression by spot level histopathology . The barplots show the mean integrated AGR2 expression by TMA spot-level histology in morphologically normal (NL), BPH, PIN, adenocarcinoma (AD), and lymph node metastases (LNMET); bars are standard errors. Levels of AGR2 expression were increased in PIN lesions and adenocarcinoma compared to BPH and morphologically normal adjacent tissue (P < 0.0001). Regional metastases showed increased AGR2 expression compared to BPH and normal tissue (P = 0.027 and P = 0.030, respectively).

Article Snippet: Slides were incubated with rabbit polyclonal anti-AGR2 antibody at 1:400 (NB110-17780, Novus Biologicals, Littleton, CO) overnight at 4°C.

Techniques: Expressing, Histopathology

Journal: BMC Cancer

Article Title: Differential expression of anterior gradient gene AGR2 in prostate cancer

doi: 10.1186/1471-2407-10-680

Figure Lengend Snippet: AGR2 expression by spot level Gleason grade . The barplots show the mean integrated AGR2 expression by TMA spot-level Gleason grade; bars are standard errors. Average AGR2 expression was increased in grades 3 and 4 compared to grade 2 (P < 0.0001 and P = 0.0007) and grade 5 (P = 0.0028 and P = 0.0085).

Article Snippet: Slides were incubated with rabbit polyclonal anti-AGR2 antibody at 1:400 (NB110-17780, Novus Biologicals, Littleton, CO) overnight at 4°C.

Techniques: Expressing

Journal: BMC Cancer

Article Title: Differential expression of anterior gradient gene AGR2 in prostate cancer

doi: 10.1186/1471-2407-10-680

Figure Lengend Snippet: Univariate Cox model

Article Snippet: Slides were incubated with rabbit polyclonal anti-AGR2 antibody at 1:400 (NB110-17780, Novus Biologicals, Littleton, CO) overnight at 4°C.

Techniques:

Journal: BMC Cancer

Article Title: Differential expression of anterior gradient gene AGR2 in prostate cancer

doi: 10.1186/1471-2407-10-680

Figure Lengend Snippet: AGR2 levels predict prostate cancer recurrence in high stage patients . Solid line is relatively higher AGR2 levels (staining measure ≥0.79); dashed line is relatively lower AGR2 levels (< 0.79). In men with stage III or IV prostate cancer, relatively lower levels of AGR2 predict a significantly greater chance of prostate cancer recurrence compared to those with higher levels (P = 0.009).

Article Snippet: Slides were incubated with rabbit polyclonal anti-AGR2 antibody at 1:400 (NB110-17780, Novus Biologicals, Littleton, CO) overnight at 4°C.

Techniques: Staining

Journal: BMC Cancer

Article Title: Differential expression of anterior gradient gene AGR2 in prostate cancer

doi: 10.1186/1471-2407-10-680

Figure Lengend Snippet: Clinicopathologic parameters and AGR2 expression in patients with high stage prostate cancer

Article Snippet: Slides were incubated with rabbit polyclonal anti-AGR2 antibody at 1:400 (NB110-17780, Novus Biologicals, Littleton, CO) overnight at 4°C.

Techniques: Expressing

Journal: BMC Cancer

Article Title: Differential expression of anterior gradient gene AGR2 in prostate cancer

doi: 10.1186/1471-2407-10-680

Figure Lengend Snippet: Multivariate Cox model

Article Snippet: Slides were incubated with rabbit polyclonal anti-AGR2 antibody at 1:400 (NB110-17780, Novus Biologicals, Littleton, CO) overnight at 4°C.

Techniques:

Journal: BMC Cancer

Article Title: Differential expression of anterior gradient gene AGR2 in prostate cancer

doi: 10.1186/1471-2407-10-680

Figure Lengend Snippet: Clinicopathologic parameters and AGR2 expression in patients with prostate cancer

Article Snippet: Slides were incubated with rabbit polyclonal anti-AGR2 antibody at 1:400 (NB110-17780, Novus Biologicals, Littleton, CO) overnight at 4°C.

Techniques: Expressing